Item Name ；Cotiform Group Enzvme substrate detection reagent
Character This product is white or light yellow particles
Clarification degree Colorless or slightly yellow
PH 7.0-7.8
Weight 2.7士 0.5 g
Storage: Long term storage, drying, sealing and avoiding light storage at 4°C – 8°C
Term of Validity 1 years
Working Principle
In the water samples containing total coliform bacteria, the target bacteria were cultured in the ONPG-MUG medium at 36 土 1 C. The specific enzyme betagalactosidase produced by the total coliform bacteria can decompose the color source substrate of the ONPG-MUG medium, which makes the culture medium yellow; meanwhile, Escherichia coli produces a specific beta-glucuronase to decompose the fluorescent substrate MUG in ONPG-MUG medium and produce characteristic fluorescence. The same principle, the heat tolerance coliform group (fecal coliform group) will decompose the color source substrate ONPG in the ONPG-MUG medium at
44.5 土 0 . 5 °C, making the medium yellow
