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Eggs Drop Syndrome1976 virus Antibody ELISA Ki

Product Code:

Item Name: Eggs Drop Syndrome1976 virus Antibody ELISA Kit

Summary: The Eggs Drop Syndrome 1976 virus(EDS76) Ab Elisa kit is use to detect specific antibody against EDS76 in serum qualitatively. For monitoring antibody after EDS76 immune and serological diagnostic of infection in Avian.

Detection Targets: Eggs Drop Syndrome1976 virus Antibody

Test Sample: Serum

Specification: 1 kit = 192 Test

Storage: All reagents should be stored at 2~8℃. Do not freeze.

Shelf Time: 12 months. Use all reagents before the expiry date on the kit.


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Eggs Drop Syndrome1976 virus Antibody ELISA Ki

Summary   Use to detect specific antibody against EDS76 in serum qualitatively.
Principle

The Eggs Drop Syndrome 1976 virus(EDS76) Ab Elisa kit is use to detect specific antibody against EDS76 in serum qualitatively. For monitoring antibody after EDS76 immune and serological diagnostic of infection in Avian.

Detection Targets  Antibody against EDS76 in serum
Sample  Serum

 

Quantity 1 kit = 192 Test
 

 

Stability and Storage

1) All reagents should be stored at 2~8℃. Do not freeze.

2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.

 

 

 

Information

Egg Drop Syndrome (EDS-76) is an acute infectious disease caused by adenovirus group III of the Adenoviridae Avian Virus genus with hemagglutination. In some chicken farms, the mass egg production of chickens suddenly decreased, and deformed eggs such as soft-shell eggs, shell-less eggs, and thin-shell eggs were produced at the same time. The whole course of the disease lasts for 5-6 weeks, after which the egg production rate gradually rises, but it is difficult to reach the level before the decline

Principle of the Test

This kit use indirect ELISA method, pured EDS76 antigen is pre-coated on enzyme micro-well strips.Whentesting,adddilutedserumsample,afterincubation,ifthereisEDS76virusspecificantibody,it will combine with the pre-coatedantigen,discardtheuncombined antibody and other components with washing; then add enzyme labled anti-EDS76 virus monoclonal antibody, then combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution 
to stop the reaction.

Contents

 

Reagent

Volume

96 Tests/192Tests

1
Antigen coated microplate

 

1ea/2ea

2
 Negative Control

 

2.0ml

3
 Positive Control

 

1.6ml

4
 Sample diluents

 

100ml

5
Washing solution (10X concentrated)

 

100ml

6
 Enzyme conjugate

 

11/22ml

7
 Substrate

 

11/22ml

8
 Stopping solution

 

15ml

9
Adhesive plate sealer

 

2ea/4ea

10 serum dilution microplate

1ea/2ea

11  Instruction

1 pcs

 


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