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Avian Leukemia P27 Antigen ELISA Kit

Product Code:

Item Name: Avian Leukemia P27 Antigen ELISA Kit

Summary: Avian Leukosis (AL) P27 antigen Elisa kit is used to detect Avian leukosis P27 antigen in avian blood, feces, cloaca, and egg white.

Detection Targets: Avian Leukemia P27 Antigen

Test Sample: Serum

Specification: 1 kit = 192 Test

Storage: All reagents should be stored at 2~8℃. Do not freeze.

Shelf Time: 12 months. Use all reagents before the expiry date on the kit.

 

 

 


Product Detail

Product Tags

Hydatid Disease Infection Antibody ELISA Kit

Summary   used to detect Avian leukosis P27 antigen in avian blood, feces, cloaca, and egg white.
Principle Avian Leukosis (AL) P27 antigen Elisa kit is used to detect Avian leukosis P27 antigen in avian blood, feces, cloaca, and egg white.

 

Detection Targets Avian Leukosis (AL) P27 antigen
Sample  Serum

 

Quantity 1 kit = 192 Test
 

 

Stability and Storage

1) All reagents should be stored at 2~8℃. Do not freeze.

2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.

 

 

 

Information

Avian Leukosis (AL) is a collective term for various tumor related diseases in poultry caused by the Avian Leukosis Virus (ALV) in the family Retroviridae. This disease is globally distributed and has a high infection rate. It can cause death and emaciation in chickens, reduce the production capacity of the flock, and is one of the main diseases that seriously endanger the development of the poultry industry. This disease has a long history and is constantly experiencing new cases, such as the Avian leukemia virus subgroup J (ALV-J), which was discovered and identified in the late 1980s in the UK as a new subtype of avian leukemia virus, causing great harm to the broiler industry

Principle of the Test

The kit uses a sandwich ELISA method,pured anti-avian leukocyte P27 monoclonal antibody is pre-coated on enzyme micro-well strips.In the test, the antigen in the sample is bound to the antibody on the coated plate, after washing to remove the unbound antigen and other components, the enzyme monoclonal antibody is added to specifically bind to the antigen-antibody complex on the test plate. then washing, the unbound enzyme conjugate is removed, TMB substrate solution is added to the microplate, the blue signal by Enzyme catalysis is directly proportion of antibody content in sample. Add stop solution, After reaction, the absorbance A value in the reaction well is measured by a wavelength of 450 nm. 

Contents

 

Reagent

Volume

96 Tests/192Tests

1
Antigen coated microplate

 

1ea/2ea

2
 Negative Control

 

2.0ml

3
 Positive Control

 

1.6ml

4
 Sample diluents

 

100ml

5
Washing solution (10X concentrated)

 

100ml

6
 Enzyme conjugate

 

11/22ml

7
 Substrate

 

11/22ml

8
 Stopping solution

 

15ml

9
Adhesive plate sealer

 

2ea/4ea

10 serum dilution microplate

1ea/2ea

11  Instruction

1 pcs

 


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